primary antibodies against human albumin  (Cell Signaling Technology Inc)

Journal: Cell Proliferation

Article Title: Isolation and characterization of stem cells from the human parathyroid gland

doi: 10.1111/j.1365-2184.2009.00614.x

Figure Lengend Snippet: Alpha‐smooth muscle actin. Red immunofluorescence staining of alpha‐smooth muscle actin (a). DAPI stains DNA blue. Parathyroid‐derived stem cells (PDSC) cultured in keratinocyte medium only (a–c) or with calcium (d,e) demonstrates alpha‐smooth mucle actin in the cytoplasm (red arrows). Scale bars: A (50 µm), B (1 µm), C (0.2 µm), D (1 µm), E (0.2 µm).

Article Snippet: For oil red O staining, cells were fixed in 3.7% formaldehyde and stained with oil red O (Sigma‐Aldrich) for 10 min. For immunofluorescence staining, cells were washed in PBS and fixed in 3.7% formaldehyde (Sigma‐Aldrich) for 15 min at room temperature, then permeabilized with 1% Triton X‐100 (Sigma‐Aldrich) for 5 min. After several washes, cells were blocked with 5% foetal bovine serum for 1 h at 37 °C, then incubated with mouse primary antibodies against alpha‐smooth muscle actin (1 : 400; Sigma‐Aldrich) or human albumin (1 : 50; Sigma‐Aldrich) for 1 h at 37 °C.

Techniques: Immunofluorescence, Staining, Derivative Assay, Cell Culture